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Wednesday, 9 March 2016

Chandrakant Ramkrishna Malwade

Chandrakant Malwade

PhD

PostDoc

University of Southern Denmark,

Odense · Department of Chemical Engineering, Biotechnology and Environmental Technology

https://www.researchgate.net/profile/Chandrakant_Malwade/info
http://findresearcher.sdu.dk/portal/files/118237294/Artemisia_poster_NNPC_2015.pdf

Niels Bohrs Allé 1
5230 Odense M
Denmark

crm@kbm.sdu.dk

Phone: 65508669

Crystallization is an inevitable step in the purification of artemisinin either from the plantArtemisia annua or from reaction mixtures of semisynthetically produced artemisinin.

Rational design of crystallization process requires knowledge about the solid–liquid equilibrium in a given solvent system and effect of impurities on it.

In the present work, a crystallization process was designed to purify artemisinin from fractions of a flash chromatography column effluent collected after injecting extracts of Artemisia annua leaves.

The fractions from chromatography containing artemisinin were combined together into one fraction, and the impurities present in this fraction were identified.

The solubility of artemisinin in the mobile phase used for chromatography, i.e.,n-hexane–ethyl acetate mixture of varying compositions, was measured at 25, 15, and 5 °C, respectively. The collective effect of impurities present in the combined fraction on the solid–liquid equilibrium of artemisinin was evaluated by measuring the solubility of artemisinin in the combined fraction at same temperatures. The results show that the impurities present in the combined fraction increase the solubility of artemisinin.

Finally, the crystallization of artemisinin from the combined fraction designed on the basis of artemisinin solubility data was carried out in two steps by adding an antisolvent and cooling crystallization.

The yield of artemisinin obtained in the process was 50%, and it was found that the impurities present in the combined fraction at a given concentration do not affect the crystallization of artemisinin.

Figure 2. Chemical structure of artemisinin (1) and impurities [artemisitene (2), dihydroartemisinic acid (3), artemisinic acid (4), arteannuin B (5), and coumarin (6)] found in the combined fraction.

Crystallization of Artemisinin from Chromatography Fractions ofArtemisia annua Extract

Chandrakant Ramkrishna Malwade^*^†, Hannes Buchholz^‡, Ben-Guang Rong^†, Haiyan Qu^†, Lars Porskjær Christensen^†, H. Lorenz^‡, and A. Seidel-Morgenstern^‡^§

^† Department of Chemical Engineering, Biotechnology and Environmental Technology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark

^‡Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, 39106 Magdeburg, Germany

^§ Institute of Process Engineering, Otto von Guericke University, 39106 Magdeburg, Germany

Org. Process Res. Dev., Article ASAP

DOI: 10.1021/acs.oprd.5b00399

Publication Date (Web): February 09, 2016

*E-mail: crm@kbm.sdu.dk; phone: 0045 65508669.

http://pubs.acs.org/doi/abs/10.1021/acs.oprd.5b00399

Conceptual process synthesis for recovery of natural products from plants: a case study of artemisinin from Artemisia annua CR Malwade, H Qu, BG Rong, LP Christensen Industrial & Engineering Chemistry Research 52 (22), 7157-7169	5	2013
Ternary solubility phase diagrams of mandelic acid and N-methylephedrine in chiral solvents with different carbon chain lengths SK Tulashie, H Lorenz, CR Malwade, A Seidel-Morgenstern Crystal Growth & Design 10 (9), 4023-4029	5	2010
Purification of artemisinin from quercetin by anti-solvent crystallization CR Malwade, H Qu, BG Rong, LP Christensen Frontiers of Chemical Science and Engineering 7 (1), 72-78	4	2013
Evaporative crystallization of carbamazepine from different organic solvents CR Malwade, H Qu, BG Rong, LP Christensen Biwic 2013: Proceedings of the 20 th Bremen International Workshop on ...		2013
Anti-solvent crystallization of artemisinin and the effect of a natural co-existing impurity CR Malwade, H Qu, BG Rong, LP Christensen Biwic 2012: Proceedings of the 19 th Bremen International Workshop on