DR ANTHONY MELVIN CRASTO,WorldDrugTracker, helping millions, A 90 % paralysed man in action for you, I am suffering from transverse mylitis and bound to a wheel chair,With death on the horizon, This will not stop me, Gods call only..........
DR ANTHONY MELVIN CRASTO Ph.D ( ICT, Mumbai) , INDIA 29Yrs Exp. in the feld of Organic Chemistry,Working for GLENMARK PHARMA at Navi Mumbai, INDIA. Serving chemists around the world. Helping them with websites on Chemistry.Million hits on google, world acclamation from industry, academia, drug authorities for websites, blogs and educational contributio
n

Monday 11 April 2016

Shaughnessy Robinson




Shaughnessy Robinson received a B.S. in Chemistry from Wright State University, OH. He worked at Sterling Winthrop and Pfizer for many years doing synthetic and computational chemistry on a variety of small molecule drug discovery programs. He is currently employed by Schrödinger, Inc. in the Drug Discovery Applications Group.






IRAK INHIBITORS AND USES THEREOF

Publication number: 20160068543
Abstract: The present invention provides compounds, compositions thereof, and methods of using the same.
Type: Application
Filed: September 17, 2015
Publication date: March 10, 2016
Inventors: Jeremy Robert Greenwood, Geraldine C. Harriman, Rosana Kapeller-Libermann, Craig E. Masse, Shaughnessy Robinson, Donna L. Romero, Mee Shelley, Ronald T. Wester
Publication number: 20160002256
Abstract: The present invention provides thienopyridine compounds, compositions thereof, and methods of using the same.
Type: Application
Filed: June 17, 2015
Publication date: January 7, 2016
Inventors: Geraldine C. Harriman, Ronald T. Wester, Donna L. Romero, Craig E. Masse, Shaughnessy Robinson, Jeremy R. Greenwood
Publication number: 20160002257
Abstract: The present invention provides arylo-fused thienopyrimidine compounds, compositions thereof, and methods of using the same.
Type: Application
Filed: June 17, 2015
Publication date: January 7, 2016
Inventors: Donna L. Romero, Shaughnessy Robinson, Matthew David Wessel, Jeremy Robert Greenwood
Patent number: 9212190
Abstract: The present invention provides compounds, compositions thereof, and methods of using the same.
Type: Grant
Filed: January 10, 2013
Date of Patent: December 15, 2015
Assignee: Nimbus Iris, Inc.
Inventors: Geraldine C. Harriman, Ronald T. Wester, Donna L. Romero, Shaughnessy Robinson, Mee Shelley, Matthew David Wessel, Jeremy Robert Greenwood, Craig E. Masse, Rosana Kapeller-Libermann
Publication number: 20150329498
Abstract: The present invention provides quinazoline and quinoline compounds, compositions thereof, and methods of using the same. Also disclosed is the activity of such compounds as inhibitors of IRAK enzymes.
Type: Application
Filed: April 21, 2015
Publication date: November 19, 2015
Inventors: Donna L. Romero, Shaughnessy Robinson, Jeremy Robert Greenwood, Mee Shelley, Craig E. Masse, Geraldine C. Harriman
Patent number: 9175007
Abstract: The present invention provides compounds, compositions thereof, and methods of using the same.
Type: Grant
Filed: January 8, 2014
Date of Patent: November 3, 2015
Assignee: Nimbus Iris, Inc.
Inventors: Jeremy Robert Greenwood, Geraldine C. Harriman, Rosana Kapeller-Libermann, Craig E. Masse, Shaughnessy Robinson, Donna L. Romero, Mee Shelley, Ronald T. Wester
Patent number: 9085586
Abstract: The present invention provides arylo-fused thienopyrimidine compounds, compositions thereof, and methods of using the same.
Type: Grant
Filed: July 11, 2013
Date of Patent: July 21, 2015
Assignee: Nimbus Iris, Inc.
Inventors: Donna L. Romero, Shaughnessy Robinson, Matthew David Wessel, Jeremy Robert Greenwood
Patent number: 9067948
Abstract: The present invention provides thienopyridine compounds, compositions thereof, and methods of using the same.
Type: Grant
Filed: July 11, 2013
Date of Patent: June 30, 2015
Assignee: Nimbus Iris, Inc.
Inventors: Geraldine C. Harriman, Ronald T. Wester, Donna L. Romero, Craig E. Masse, Shaughnessy Robinson, Jeremy Robert Greenwood
Publication number: 20150094305
Abstract: The present invention provides compounds, compositions thereof, and methods of using the same.
Type: Application
Filed: September 25, 2014
Publication date: April 2, 2015
Inventors: Donna ROMERO, Craig E. MASSE, Shaughnessy ROBINSON, Jeremy Robert GREENWOOD, Geraldine C. HARRIMAN
Publication number: 20150025093
Abstract: The present invention provides compounds, compositions thereof, and methods of using the same.
Type: Application
Filed: February 25, 2014
Publication date: January 22, 2015
Applicant: Nimbus Iris, Inc.
Inventors: Donna L. Romero, Matthew David Wessel, Shaughnessy Robinson, Jeremy Robert Greenwood, Karl Shawn Watts, Leah Lynn Frye, Geraldine C. Harriman, Alan Franklin Corin, Craig E. Masse, Mee Shelley
Publication number: 20140194417
Abstract: The present invention provides compounds, compositions thereof, and methods of using the same.
Type: Application
Filed: January 8, 2014
Publication date: July 10, 2014
Applicant: Nimbus Iris, Inc.
Inventors: Jeremy Robert Greenwood, Geraldine C. Harriman, Rosana Kapeller-Libermann, Craig E. Masse, Shaughnessy Robinson, Donna L. Romero, Mee Shelley, Ronald T. Wester
Patent number: 8703941
Abstract: The present invention provides thienopyrimidine and thienopyridine compounds, compositions thereof, and methods of using the same for the treatment of diseases mediated by IRAK enzymes. Such diseases include inflammatory and proliferative diseases.
Type: Grant
Filed: January 10, 2012
Date of Patent: April 22, 2014
Assignee: Nimbus Iris, Inc.
Inventors: Donna L. Romero, Matthew David Wessel, Shaughnessy Robinson, Jeremy Robert Greenwood, Karl Shawn Watts, Leah Lynn Frye, Geraldine C. Harriman, Alan Franklin Corin, Craig E. Masse, Mee Shelley
Publication number: 20140018343
Abstract: The present invention provides arylo-fused thienopyrimidine compounds, compositions thereof, and methods of using the same.
Type: Application
Filed: July 11, 2013
Publication date: January 16, 2014
Inventors: Donna L. Romero, Shaughnessy Robinson, Matthew David Wessel, Jeremy Robert Greenwood
Publication number: 20140018357
Abstract: The present invention provides thienopyridine compounds, compositions thereof, and methods of using the same.
Type: Application
Filed: July 11, 2013
Publication date: January 16, 2014
Inventors: Geraldine C. Harriman, Ronald T. Wester, Donna L. Romero, Craig E. Masse, Shaughnessy Robinson, Jeremy Robert Greenwood
Publication number: 20140018361
Abstract: The present invention provides furano- and pyrrolo-pyrimidine and pyridine compounds, compositions thereof, and methods of using the same.
Type: Application
Filed: July 11, 2013
Publication date: January 16, 2014
Inventors: Geraldine C. Harriman, Donna L. Romero, Craig E. Masse, Shaughnessy Robinson, Matthew David Wessel, Jeremy Robert Greenwood
Publication number: 20130231328
Abstract: The present invention provides compounds, compositions thereof, and methods of using the same.
Type: Application
Filed: January 10, 2013
Publication date: September 5, 2013
Applicant: NIMBUS IRIS, INC.
Inventors: Geraldine C. Harriman, Ronald T. Wester, Donna L. Romero, Shaughnessy Robinson, Mee Shelley, Matthew David Wessel, Jeremy Robert Greenwood, Craig E. Masse, Rosana Kapeller-Libermann
Publication number: 20120283238
Abstract: The present invention provides compounds, compositions thereof, and methods of using the same.
Type: Application
Filed: January 10, 2012
Publication date: November 8, 2012
Applicant: NIMBUS IRIS, INC.
Inventors: Donna L. Romero, Matthew David Wessel, Shaughnessy Robinson, Jeremy Robert Greenwood, Karl Shawn Watts, Leah Lynn Frye, Geraldine C. Harriman, Alan Franklin Corin, Craig E. Masse
Publication number: 20120065188
Abstract: Compounds of the general Formula I, wherein X1, X2, X3, X4, X5, X6, X7, R1, R2, R4, R5, R6, R7, R8, R9, R10, Y1, n, m, p and q are defined as above, their preparation and their use as antimicrobial agents.
Type: Application
Filed: November 22, 2011
Publication date: March 15, 2012
Inventors: Steven Joseph Brickner, Jinshan Michael Chen, Zhengong Bryan Li, Anthony Marfat, Mark Joseph Mitton-Fry, Michael A. Plotkin, Usa Datta Reilly, Chakrapani Subramanyam, Zhijun Zhang, Shaughnessy Robinson
Publication number: 20110092480
Abstract: Compounds of the general Formula I, wherein X1, X2, X3, X4, X5, X6, X7, R1, R2, R4, R5, R6, R7, R8, R9, R10, Y1, n, m, p and q are defined as above, their preparation and their use as antimicrobial agents.
Type: Application
Filed: December 20, 2010
Publication date: April 21, 2011
Inventors: Steven Joseph Brickner, Jinshan Michael Chen, Zhengong Bryan Li, Anthony Marfat, Mark Joseph Mitton-Fry, Michael A. Plotkin, Usa Datta Reilly, Chakrapani Subramanyam, Zhijun Zhang, Shaughnessy Robinson
Publication number: 20080280879
Abstract: Compounds of the general Formula I, wherein X1, X2, X3, X4, X5, X6, X7, R1, R2, R4, R5, R6, R7, R8, R9, R10, Y1, n, m, p and q are defined as above, their preparation and their use as antimicrobial agents.
Type: Application
Filed: May 8, 2008
Publication date: November 13, 2008
Inventors: Steven Jospeh BRICKNER, Jinshan Michael Chen, Zhengong Bryan Li, Anthony Marfat, Mark Joseph Mitton-Fry, Micheal A. Plotkin, Usa Datta Reilly, Chakrapani Subramanyam, Zhijun Zhang, Shaughnessy Robinson 
 
 












Recent Advances in the Discovery of Small Molecule Inhibitors of Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) as a Therapeutic Target for Inflammation and Oncology Disorders

Miniperspective

 Nimbus Discovery, 25 First Street, Suite 404, Cambridge, Massachusetts 02141, United States
 Schrödinger Inc., 120 West Forty-Fifth Street, New York, New York 10036, United States
J. Med. Chem.201558 (1), pp 96–110
DOI: 10.1021/jm5016044

Divya Chaudhary



 Divya Chaudhary

Divya Chaudhary

Pharmaceutical R&D and Commercial Strategy

 links
 https://www.linkedin.com/in/divya-chaudhary-7584579
 https://twitter.com/divya_divyach
https://www.researchgate.net/profile/Divya_Chaudhary3


Divya Chaudhary has been working in the pharmaceutical and biotechnology industry in drug discovery and development of small molecules and biologics for autoimmune diseases and oncology at Wyeth, Pfizer, and Nimbus Discovery. She received her Ph.D. from University of Alabama at Birmingham at the Comprehensive Cancer Center, working on regulation of oncogene expression, and did postdoctoral fellowships at University of Michigan and Genentech in molecular oncology and signal transduction.


Divya Chaudhary

Summary

Created value in pharmaceutical R&D organizations in small molecule and biologics drug discovery and development. Strong experience in strategic thinking and innovation, communication and diligence, and effective management of projects with diverse functional teams.

 Effectivey managed external partners and client relations
 Mentored staff as they enter and grow in organizations
 Unique combination of a deep scientific and a strategy consulting experience

Disease areas experience in inflammation, autoimmune diseases, respiratory diseases, and immuno-oncology

Specialized in commercialization and operations strategy in life sciences, pharmacology, biochemistry, cell biology, CRO and supply chain, and program and alliance management

Experience


Consultant

IMS Health
– Present (less than a year)
Managed Markets Strategy for Commercialization of Bio-Pharmaceutical Products
Conducted subnational market analysis and created Tableau dashboard tools for a newly launched therapeutic to help define local market strategies

Life Science Consultant

Putnam Associates
(1 year 4 months)
 Conducted primary research with physicians, academics, and key opinion leaders for development insights and commercial assessments for multiple assets
 Analyzed physician and patient data to understand segmentation/ positioning and building patient journey and treatment flow models
 Refined a revenue model by performing sensitivity analysis, for the purpose of projecting revenue scenarios
 Conducted due diligence for private equity client evaluating a medium sized pharmaceutical company’s assets
 Developed product positioning strategies for cell immunotherapy product in hematology
 Assessed global opportunities in vaccines; conducted research for HEOR analysis data inputs for the purpose of asset prioritization

Head of Pharmacology

Nimbus Discovery
(3 years)
Led preclinical discovery in a virtual CRO outsourced model, contributing to successful series B and program transaction
 Contributed to the selection of IRAK4 inhibitor development candidates resulting in program partnering with Genentech
 Communicated lead candidate profiles and timelines for board meetings and external discussions, and for diligence discussions and evaluation
 Initiated and fast tracked the oncology project strategy with staff embedded at a CRO

Managed the early efforts to initate the ACC inhibitor evaluation in tumor cell lines
 Developed the preclinical markers approach working with a CRO to contribute to the program's oncology strategy

Advanced critical business development objectives in the areas of cancer and autoimmune disease
 Evaluated and critiqued multiple target ideas; initiated and implemented two programs
 Proposed, planned, and led TYK2 inhibitor lead optimization biology effort

Writer

Biopharmaceutical R&D
(6 months)
Performed writing work for bio-pharmaceutical company

Consultant

Nimbus Discovery
(6 months)
Project management for IRAK4 inhibitor and ACC inhibitor programs lead discovery efforts, with contract research organization, for both fee for service and full time equivalent resourced projects

Senior Principal Scientist

Pfizer Pharmaceuticals
(2 years)
Managed the preclinical, biomarker, and pharmacology strategy for four autoimmune disease programs (small molecule and biologics)

Scientist

Wyeth Research
(11 years)
Managed and led two biologics programs and one small molecule program

Postdoctoral Fellow

Genentech
(1 year)





Education


Northeastern University

MBA

Activities and Societies: Beta gamma sigma

Indian Institute of Technology, Roorkee

M.Sc., BioSciences and Biotechnology

Graduated top of the class with a gold medal award
Activities and Societies: RUSA Senator

University of Alabama at Birmingham - School of Medicine

Ph.D., Biochemistry and Molecular Genetics


















Recent Advances in the Discovery of Small Molecule Inhibitors of Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) as a Therapeutic Target for Inflammation and Oncology Disorders

Miniperspective

 Nimbus Discovery, 25 First Street, Suite 404, Cambridge, Massachusetts 02141, United States
 Schrödinger Inc., 120 West Forty-Fifth Street, New York, New York 10036, United States
J. Med. Chem.201558 (1), pp 96–110
DOI: 10.1021/jm5016044

Subrahmanyam Vangala

 

Subrahmanyam Vangala

Advinus Therapeutics
USA links
 https://www.researchgate.net/profile/Subrahmanyam_Vangala2
 https://twitter.com/subrahmv
 https://www.linkedin.com/in/subrahmanyamvangala
 https://www.facebook.com/subrahmanyam.vangala
  • Drug Metabolism, Pharmacokinetics, Clinical Pharmacology & Investigative Toxicology
    Bengaluru, India
SubrahmanyamVangala is Vice President, DMPK, Clinical Pharmacology & Investigative Toxicology at Advinus Therapeutics in Bangalore, India. He has 22 years of pharmaceutical industry experience at major pharmaceutical companies in USA including American Cyanamid/Wyeth, Purdue Pharma (PP), JNJ and Shire. He returned to India in 2008 as Vice President of DMPK and Toxicology at Sai Advantium Pharma (SAP). He built DMPK departments ground up at PP and JNJ. At SAP, he built the infra-structure for DMPK & Toxicology groups for integrated drug discovery support of Medicinal Chemistry programs. For the last 16 years he managed DMPK, Toxicology and Pharmacology groups at various capacities.
Dr. Subrahmanyam received his PhD (1987) in Molecular Toxicology from Department of Biochemistry at Memorial University, Canada. Following PhD, he completed 2 year postdoctoral training in the Department of Molecular Toxicology at University of Colorado and heldResearch Assistant Professor Position in the School of Public Health at University of California, Berkeley for 3 years, before joining the industry. He chaired the inaugural Land-O’-Lakes Drug Metabolism and Applied Pharmacokinetics Conference(1998) and 1st and 2nd Idiosyncratic Drug toxicity conferences (1998 and 2000). He gave guest lecturers as invited speaker at several international conferences related to ADME/Predictive toxicology.  In 2005 he received Excellence in Science Award from JNJ. Since 2010 serving as editor of the STM Journal of Toxicology: Research and Reviews.Served on international advisory board for journal Current Pharmacogenomics and Personalized Medicine (CPPM-2012 & 2013). He published ~40 research articles and reviews including several invited book chapters.

Research Interest

Drug discovery & development relating to DMPK; Clinical pharmacology; Investigative toxicology; Biomarkers and molecular diagnostics.




Research Experience

  • Jun 2013–present
    Vice President
    Advinus Therapeutics · Drug Metabolism, Pharmacokinetics, Clinical Pharmacology, Genetic Toxicology & Eco Toxicology
    India · Bangalore
  • Jun 2012–May 2013
    Manager/Partner
    Ridgeview Clinical Consulting
    Ringoes, New Jersey
  • Jan 2008–May 2012
    Vice President
    Sai Advantium Pharma Ltd. · Pharmacology & Toxicology · Senior Management
    India · Hyderābād
  • Dec 2006–Dec 2007
    Director
    Shire Human Genetic Therapies · Toxicology & Pharmacology
    USA · Lexington
  • Sep 2001–Nov 2006
    Site Head/Research Fellow
    Johnson & Johnson · Pharmacokinetics and Drug Metabolism
    USA · Raritan, New Jersey
  • Sep 1992–Jan 1998
    Senior Scientist
    American Cyanamid Company/Lederle Laboratories/Wyeth Research · Pharmacokinetics, Pharmacodynamics, Biotransformation
    USA · Pearl River New York and Princeton, New Jersey
  • Jan 1989–Aug 1992
    University of California, Berkeley · School of Public Health
    USA · Berkeley
  • Feb 1988–
    Aug 2001
    Group Leader
    Purdue Pharma L.P. · Pharmacokinetics and Drug Metabolism
    USA · Ardsley, New York
  • Jan 1987–
    Dec 1988
    University of Colorado at Boulder · Molecular Toxicology at School of Pharacy
    USA · Boulder, Colorado
  • Jan 1986–Dec 1986
    University of Toronto
    University of Toronto, School of Medicine · Biochemistry and Pathology
    Canada · Toronto

Education

  • Jan 1981–Dec 1985
    Memorial University of Newfoundland
    Biochemistry of Cancer · PhD
    Canada · St. John's, Newfoundland

Summary

Experienced professional who excels in both scientific and regulatory expertise for drug discovery and development success. Strong track record of accomplishment in building infra-structures and integrating departments into effective, value-added support for R&D organizational success..
Primary areas of expertise include Toxicology, Pharmacology, DMPK, Bioanalytical, Clinical Pharmacology and Investigative/Mechanistic Toxicology. Experienced in both small molecule and biotechnology-based drug platforms. Some experience with large molecule drug development. Therapeutic areas of expertise include CNS, Neuro-degenerative diseases, Metabolic disorders, Inflammation, Oncology, Anti-infectives and Women's Health (eg, SERMs).

Experience


Manager/Partner

Ridgeview Clinical
– Present (4 years)Ringoes, New Jersey

Vice President, Pharmacology & Toxicology

Sai Advantium Pharma Ltd, Pune India
(4 years 6 months)Pune Area, India
In the last 4 years of my tenure at SAI, I established the infra structure for drug discovery support services relating to Pharmacology, ADME and Toxicology screening. Designed the laboratories that included 20,000 SF, hired about 40 plus scientists (including 5 Associate Directors as group managers), and established validated protocols for ADMET services,

Supported ADMET services for several biotech companies in US and Europe.

The animal facilities are accredited by Indian CPCSEA and American AAALAC authorities. Selected areas of in vitro and in vivo laboratories are also approved for conducting radiolabel studies. ADME services include in vivo PK in rodents, mass balance, tissue distribution, protein binding, caco-2/mdck permeability, CYP profiling (competitive inhibition,time-based inhibition, phenotyping), metabolic stability, profiling & metabolite ID, GSH-trapping of reactive intermediates, physico-chemical properties (solubility, stability, pka, logP, logD).
Toxicology services include: acute, DRF, pivotal one month tox, (includes: clinical observations, clinical pathology, hematology, histopathology, toxicokinetics), genetic toxicology (AMES, micronucleus tests).

Pharmacology services include radioligand binding assays, cell based assays, in vivo models of pharmacology (pain, neurodegenerative diseases and inflammation)

For a smooth operational excellence, appropriate processes are put in place to avoid delays and ensure high quality and timely delivery of results and reports to the clients.

Communication excellence of all employees is considered number one piority and appropriate training is provided for all departmental personnel.

Safe laboratory practices has been made mandatory with appropriate training recommendations are made for employees by "Health & Safety Environment" department.

Budget projections based on business needs and for strategic growth are provided to senior executives with rational justifications.

Director, Toxicology and Pharmacology

Shire HGT
(1 year 1 month)Cambridge, Massachussettes
Provided PK and Toxicology support for "Enzyme Replacement Therapy" programs. Managed outsourcing of all PK and Toxicology studies. Designed and reviewed protocols, analyzed PK and Toxicology data, reviewed reports, presented at Senior management meetings. Projected quarterly budgets and provided appropriate justifications. During this job, I had an opportunity to learn drug discovery/development processes and associated regulatory challenges for large molecule biologics.

Site Director/Research Fellow, Drug Metabolism and Pharmacokinetics

Johnson & Johnson
(5 years 2 months)Raritan, New Jersey
•Built a strong DMPK department from a size of 4 to 28 scientists (Total of 7 PhD: 4 direct reports (2 GL, 1 RS and 1 PS).
•Included experts in compartmental & non-compartmental methods of PK/TK (GLP), allometric scaling, IV/IVC of ADME, metabolite ID (LC/MS), in vitro DDI, absorption, transporter, biliary excretion studies. Evaluation of novel technologies (metabonomics, reactive metabolites, RED for protein binding).
•Established a competitive DMPK Dept, attracting the best talent from major Pharma including Merck, Aventis, Pfizer, Schering, Roche.
•Participated in project teams for preclinical development. Provided preclinical strategies, proposals, and timelines. Provided critical, expert input in go/no go decisions.
•Managed annual DMPK budget of up to 8 MM (USD). Prepared budgets, short/long-term strategies, requested appropriate human and capital resources for dept growth. Balanced in-house and out-sourced activities, with >90% in-house capacity by 2006 compared to <10% in 2001. Justified the purchase of LTQ-Orbitrap to Senior management and finance department and established the state-of-the-art technology for DM studies.
• Active and lead role in the global harmonization of CYP-DDI assays. Identified similarities and differences in processes, assay methods for DDI studies at other JNJ companies. Where needed, harmonized assay protocols in consensus with other DMPK leaders at JNJ.
•Collaborated with Mechanistic Toxicology group to provide DMPK support to address specific project related safety issues. Key management representative for “Hepatotoxicity Expert Group” for evaluation of hepatotoxicity mechanisms.
•Delegated resources to collaborate with CATD (Computer Aided Trial Design) group for PK-PD model development using preclinical data.
•Participated in due-diligence teams for in-licensing candidates by reviewing the Preclinical data packages.
•Successfully completed year long “Management Fundamentals” training course at JNJ.

Section Head/Group Leader, Drug Metabolism and Pharmacokinetics

Purdue Pharma
(3 years 6 months)Ardsley, Westchester, New York
•Managed 8–10 scientists including 4 PhD’s. Established drug metabolism laboratory that was non-existent at Purdue. Managed the PKDM group, supported discovery and development projects- including in vitro/in vivo DM, in vitro DDI, writing preclinical sections for NDA/IND.
•Represented the project teams and sub-teams for DMPK, toxicology, bioanalytical, activities.
•Established hepatocyte cell culture methods, developed cryopreserved hepatocytes banks and validated their use for drug metabolism and CYP induction studies.
•Established HPLC/MS coupled to radiometric detection for metabolite separation and identification to support preclinical and clinical programs.
•Established high throughput screening methods for discovery support - Fluorescence cytotoxicity assays using hepatocytes, bDNA assay for cytochrome P450 induction in hepatocytes.
•Established CYP enzyme ID methods using - correlation analysis, recombinant enzymes and specific inhibitor assays. Developed and validated the use of characterized bank (16 subjects) of human liver microsomes for CYP ID studies.
•Established drug interaction studies using microsomes, recombinant enzymes and hepatocytes. Made recommendations for clinical drug interaction program. Established high-throughput fluorescence P450 inhibition screens for discovery support, Coordinated mass-spec activities for metabolite identification.
•Played key role in help building a Discovery Support program that was non-existent at Purdue. Provided strategies on how a discovery support group could help in lead selection and optimization. Identified industry experts in heading this group.
•Coordinated contract laboratory activities for drug metabolism studies in hepatocytes/microsomes, CYP enzyme ID, induction, inhibition, genotyping and Caco-2 permeability studies.
•Established collaborative efforts with academia for assessing mechanisms of drug toxicity using cellular models and selecting lead candidates from backups.

Senior Scientist 1 & 2

American Cyanamid/Wyeth
(5 years 6 months)Pearl River, New York (AMC) & Princeton, New Jersey (Wyeth)
•As a principal investigator, conducted extensive hands-on toxicokinetics analysis in accordance with GLP. Supported all non-GLP and GLP toxicology including acute, sub-chronic and chronic studies.
•As a study director and principal investigator designed and conducted PK (Bioavailability), mass-balance, protein binding, in vivo/in vitro DM, (CYP isozyme ID, CYP-DDI) studies.
-Conducted hands-on investigative studies to elucidate the mechanisms underlying long plasma 14C-half life (>22 days) of a drug candidate. Collaborated with Analytical Development group to conduct amino acid analysis of plasma proteins and with academia to urinalysis of endogenous metabolic products. The investigations revealed a novel mechanism of one-carbon recycling of carbon released from the drug and incorporation into amino acids, proteins and nucleic acids.

-Conducted hands-on studies and evaluated non-CYP mediated phase-1 and phase 2 DM for selected preclinical drug candidates based on strucutre-activity relations. These enzymes include molybdenum hydroxylases (aldehyde and xanthine oxidses), monoamine oxidases, carboxylesterases, amidases and UDP-glucuronosyl trnsferases.
-Established recombinant human (rh) CYP-isozyme banks (from cDNA expressed E.coli cells) for routine drug metabolism studies. Validated the rhCYP isoforms using standard probe substrates and HPLC analysis.
-Participated in LC-NMR evaluation in collaboration with Bruker for drug metabolism and metabolite-ID studies.
-Supervised two technical assistants. Prepared reports and DM sections of IND/NDA sections for regulatory submissions.

Senior Scientist

American Cyanamid
(6 years)

Senior Scientist

American Cyanamid
(6 years)

Senior Scientist

American Cyanamid
(6 years)

Research Assistant Professor

UC Berkeley
(3 years 8 months)Berkeley, California
-Supervised a group of four scientists (1 PhD and 3 graduate students).

-Designed in vitro and in vivo metabolism and molecular toxicology studies using isolated enzyme systems (microsomes, recombinant CYP enzymes, prostaglandin H synthase, myeloperoxidase etc), cell cultures (HL-60 cells, human lymphocytes) and in vivo mouse models.

-Established measurement of oxidative DNA damage techniques for assessing non-genotoxic events in chemical carcinogenesis using HL-60 cells and mouse models (Collaborated with Dr. Bruce Ames laboratory at Department of Biochemistry for measurement of oxidative DNA damage by HPLC-Electrochemical detection methods).

-Conducted mechanistic drug metabolism studies using hemeprotein peroxidases. Investigated novel cooperative interactions of heme and apoprotein in catalyzing drug metabolism.

-Lectured “Chemical Carcinogenesis” course to graduate students.

Postdoctoral Research Associate, Envoronmental and Molecular Toxicology, School of Pharmacy

University of Colorado at Boulder
(2 years)Boulder, Colorado
Conducted hands-on in vitro drug metabolism and molecular toxicology experiments utilizing hepatocytes. Mastered in-situ liver perfusion techniques for isolating hepatocytes from rats. Developed techniques for measuring various biochemical and molecular end- points of cell death (GSH depletion, ATP depletion, inhibition of protein and RNA synthesis, covalent binding to proteins and DNA etc.).

Conducted Drug Metabolism and toxicity studies in bone marrow homogenates and bone marrow stromal cells. Evaluated role of cytokines and other growth factors in bone marrow toxicity

Supervised one technician and one graduate student.








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